With a vision “to drive responsible transformation for regions and communities, serve them with creative solutions by joint outcomes from incorporating research into education and turn contemporary challenges of our era into opportunities”, seven leading universities unite in their pledge to establish the INVEST - INnoVations of REgional Sustainability European university Alliance. We are committed into a deeper, sustainable and an effective transnational cooperation that promotes European Identity and European values leading to sustainable, short- and long-term impacts. With this premise, our aim is to advance knowledge, increase the quality and relevance of higher education and research, strengthen links between education, research, and innovation, improve employability and skills, make more effective use of digital technologies and open science, towards imparting inclusive education and training at all levels. We shall achieve this based on three strategic pillars: (a) Regional Transformation, Ecosystems & Entrepreneurship; (b) Flexible education & work-based pedagogy; (c) Developing a Centre of Excellence in RDI. Our main focus areas are Water, Energy, Food and Environment Nexus, Quality of Life and Entrepreneurship. We intensify the use of our Living Labs as innovative platforms for quadruple helix collaboration among research, education, innovation and service to society. The process is supported by innovative digital solutions such as the Virtual Campus and the EDUC8EU platforms. The INVEST Alliance endeavours to meet the needs and requirements of the new generation of Europeans who are able to cooperate and work within different European and global cultures, in different languages, across borders, sectors, academic disciplines, and respond to the current global challenges determined within the UN Sustainable Development Goals. INVEST will strive impact and create models for good practices for a more sustainable higher education and a more sustainable Europe.